Blood and Blood-derived Therapeutics

• Nemkov T, Sun K, Reisz JA, Song A, Yoshida T, Dunham A, Wither MJ, Francis RO, Roach RC, Dzieciatkowska M, Rogers SC, Doctor A, Kriebardis A, Antonelou M, Papassideri I, Hansen KC, Spitalnik SL, Xia Y, Zimring JC, Hod EA, D’Alessandro A*. Hypoxia modulates the purine salvage pathway and decreases cell and supernatant levels of hypoxanthine, a predictor of 24h in vivo survival of stored mouse and human red blood cells. Haematologica 2017;  doi: 10.3324/haematol.2017.178608. *corresponding author

• Nemkov T, Hansen KC, D’Alessandro A* Three Minute Method for high-throughput quantitative metabolomics and quantitative flux-analysis of central carbon and nitrogen pathways. Rapid Comm Mass Spectrometry 2017; 31(8):663-673.  *corresponding author

• D’Alessandro A, Liumbruno GM. Editorial. Red blood cell storage and clinical trials. Blood Transfusion 2017; 15(2):101-103.

• D’Alessandro A, Seghatchian J. Hitchhiker’s guide to the red blood cell storage galaxy. Transfusion Science 2017; 56(2):248-253.

• D’Alessandro A, Zolla L. Red blood cell proteomics for clinical applications. Exp Rev Prot 2017; 14(3):243-252. *corresponding author

• D’Alessandro A, Nemkov T, Reisz J, Dzieciatkowska M, Wither MJ, Hansen KC. Omics markers of the red cell storage lesion and metabolic linkage. Blood Transfusion 2017; 15(2):137-144.

• D’Alessandro A, Szczepiorkowski Z, Gray AD, Dumont LJ. Metabolomics of outdated red blood cells upon rejuvenation-freezing/thawing/deglycerolization and 4C overnight hold after 24h refrigerated storage. Transfusion 2017; 57(4):1019-1030. 

• Song A, Zhang Y,  Han L, Yegutkin GG, Liu H, Sun K, D’Alessandro A, Lee J, Karmouty-Quintana H, Iriyama T, Weng T, Zhao S, Wang W, Wu H, Hansen KC, Zhang H, Bogdanov M, Dowhan W,  Jin J, Kellems RE, Eltzschig HK, Blackburn M, Roach RC0, Xia Y. Erythrocytes retain “hypoxic purinergic memory” for faster acclimatization upon re-ascent. Nat Comm 2017; 8:14108.

• Reisz JA, Chessler KM, Dzieciatkowska M, D’Alessandro A*, Hansen KC. Blood and plasma proteomics: targeted quantitation and post-translational redox modifications. Meth Mol Biol 2017; 1619:353-371. *corresponding author

• D’Alessandro A, Dzieciatkowska M, Nemkov T, Hansen KC. A Red blood cell proteomics update- is there more to discover? Blood Transfusion 2017; 15(2):182-187. 

• D’Alessandro A*, Nemkov T, Yoshida T, Bordbar A, Palsson BO, Hansen KC, Quantitative metabolic flux analysis of red blood cells stored in Additive Solution-3 reveals unappreciated metabolism of other substrates than glucose. Transfusion 2017; ;57(2):325-336. *corresponding author

• D’Alessandro A* Red blood cell storage lesion. Vox Sang ISBT Series 2016; DOI: 10.1111/voxs.12298 *corresponding author

• Paglia G, D’Alessandro A, Sigurjonsson O, Rolfsson O, Bordbar A, Gudmondsson S, Nemkov T, Hansen KC, BO. Biomarkers defining the metabolic age of red blood cells during cold storage. Blood 2016;  128(13):e43-50.

• Reisz JA, Wither M, Dzieciatkowska M, Issaian A, Hansen KC, D’Alessandro A*. Metabolic regulation through oxidation/ vesiculation of glyceraldehyde 3-phosphate dehydrogenase in red blood cells stored under blood bank conditions in additive solution 3. Blood 2016; 128(12):e32-42. *corresponding author

• Tzounakas VL, Kriebardis AG, Georgatzakou H, Foudoulaki-Paparizos LE, Dzieciatkowska M, Wither MJ, Nemkov T, Hansen KC, Papassideri I, D’Alessandro A,# and Marianna H. Antonelou#. Data on how several physiological parameters of stored red blood cells are similar in glucose 6-phosphate dehydrogenase deficient and  sufficient donors. Free Rad Biol Med 2016; ;8:618-27. d *corresponding author

• Liu H, Zhang Y, Song A, Yegutkin GG, D’Alessandro A, Lee J, Cheng NY, Sun H, Kellems RE, Karmouty-Quintana H, Wen TT, Subudhi AW, Jameson-Van Houten S, Julian CG, Lovering AT, Eltzschig HK, Blackburn MK,  Nemkov T, Hansen KC, Roach RC, Xia Y. Adenosine signaling via AMPK-induced erythrocyte oxygen release: a key mechanism for human hypoxia adaptation and novel therapy. Circulation 2016; ;134(5):405-21

• Sun K*, Zhang Y*, D’Alessandro A*, Song A, Wu H, Liu H, Adebiyi M, Huang A, Wen YE, Bogdanov MV, Nemkov T, Hansen KC, Kellems RE, Dowhan W, Subudhi AW, Jameson-Van Houten S, Julian CG, Lovering AT, Vila A, O’Brien J, Roach RC, Yang X. Erythrocyte sphingosine 1 phosphate: a key intracellular modulator for adaptation to high altitude hypoxia. Nature Comm 2016; 7:12086.  *shared first authorship

• Tzounakas VL, Kriebardis AG, Georgatzakou H, Foudoulaki-Paparizos LE, Dzieciatkowska M, Wither MJ, Nemkov T, Hansen KC, Papassideri I, D’Alessandro A,# Antonelou MH#. Storage of glucose 6-phosphate dehydrogenase deficient red blood cells .  Free Rad Biol Med 2016; 96:152-65.. #corresponding authors

• Nemkov T, Hansen KC, Ness P, Dumont LJ, D’Alessandro A*. Metabolomics in Transfusion Medicine. Transfusion 2016; 56(4):980-93. * corresponding author

Extracellular Matrix and Regenerative Medicine

• Liu S, Li X, Zhang L, Jiang J, Hill RC, Cui Y, Hansen KC, Zhou ZH, Zhao R. Structure of the yeast spliceosomal postcatalytic P complex. Science. 2017 Nov 16. pii: eaar3462. doi: 10.1126/science.aar3462. [Epub ahead of print] PubMed PMID: 29146870.

• Li X, Liu S, Jiang J, Zhang L, Espinosa S, Hill RC, Hansen KC, Zhou ZH, Zhao R. CryoEM structure of Saccharomyces cerevisiae U1 snRNP offers insight into alternative splicing. Nat Commun. 2017 Oct 19;8(1):1035. doi:10.1038/s41467-017-01241-9. PubMed PMID: 29051543; PubMed Central PMCID: PMC5648754.

• Barrett AS, Wither MJ, Hill RC, Dzieciatkowska M, D'Alessandro A, Reisz JA, Hansen KC. Hydroxylamine Chemical Digestion for Insoluble Extracellular Matrix Characterization. J Proteome Res. 2017 Nov 3;16(11):4177-4184. doi 10.1021/acs.jproteome.7b00527. PubMed PMID: 28971683.

• Hill RC, Oman TJ, Wang X, Shan G, Schafer B, Herman RA, Tobias R, Shippar J, Malayappan B, Sheng L, Xu A, Bradshaw J. Development, Validation, and Interlaboratory Evaluation of a Quantitative Multiplexing Method To Assess Level of Ten Endogenous Allergens in Soybean Seed and Its Application to Field Trials Spanning Three Growing Seasons. J Agric Food Chem. 2017 Jul 12;65(27):5531-5544. doi: 10.1021/acs.jafc.7b01018. Epub 2017 Jun 29. PubMed PMID: 28635260.

• Goddard ET, Hill RC, Nemkov T, D'Alessandro A, Hansen KC, Maller O, Mongoue-Tchokote S, Mori M, Partridge AH, Borges VF, Schedin P. The Rodent Liver Undergoes Weaning-Induced Involution and Supports Breast Cancer Metastasis Cancer Discov. 2017 Feb;7(2):177-187. doi: 10.1158/2159-8290.CD-16-0822. Epub 2016 Dec 14. PubMed PMID: 27974414; PubMed Central PMCID: PMC5459606.

• Goddard ET, Hill RC, Barrett A, Betts C, Guo Q, Maller O, Borges VF, Hansen KC, Schedin P. Quantitative extracellular matrix proteomics to study mammary and liver tissue microenvironments. Int J Biochem Cell Biol. 2016 Dec;81(PtA):223-232. doi: 10.1016/j.biocel.2016.10.014. Epub 2016 Oct 24. PubMed PMID: 27771439; PubMed Central PMCID: PMC5459605.

• Calle EA, Hill RC, Leiby KL, Le AV, Gard AL, Madri JA, Hansen KC, Niklason LE. Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices. Acta Biomater. 2016 Dec;46:91-100. doi: 10.1016/j.actbio.2016.09.043. Epub 2016 Sep 29. PubMed PMID:27693690; PubMed Central PMCID: PMC5451113.

• Laklai H, Miroshnikova YA, Pickup MW, Collisson EA, Kim GE, Barrett AS, Hill RC, Lakins JN, Schlaepfer DD, Mouw JK, LeBleu VS, Roy N, Novitskiy SV, Johanse JS, Poli V, Kalluri R, Iacobuzio-Donahue CA, Wood LD, Hebrok M, Hansen K, Mose, HL, Weaver VM. Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression. Nat Med. 2016 May;22(5):497-505. doi: 10.1038/nm.4082. Epub 2016 Apr 18. PubMed PMID: 27089513; PubMed Central PMCID: PMC4860133.

• D'Alessandro A, Dzieciatkowska M, Hill RC, Hansen KC. Supernatant protein biomarkers of red blood cell storage hemolysis as determined through an absolute quantification proteomics technology. Transfusion. 2016 Jun;56(6):1329-39. doi:10.1111/trf.13483. Epub 2016 Jan 26. PubMed PMID: 26813021.

• Johnson TD, Hill RC, Dzieciatkowska M, Nigam V, Behfar A, Christman KL, Hansen KC. Quantification of decellularized human myocardial matrix: A comparison of six patients. Proteomics Clin Appl. 2016 Jan;10(1):75-83. doi:10.1002/prca.201500048. Epub 2015 Sep 28. PubMed PMID: 26172914; PubMed Central PMCID: PMC4703489.

• Dzieciatkowska M, D'Alessandro A, Hill RC, Hansen KC. Plasma QconCATs reveal a gender-specific proteomic signature in apheresis platelet plasma supernatants. J Proteomics. 2015 Apr 29;120:1-6. doi: 10.1016/j.jprot.2015.02.010. Epub 201 Mar 2. PubMed PMID: 25743772.

• Hill RC, Calle EA, Dzieciatkowska M, Niklason LE, Hansen KC. Quantification of extracellular matrix proteins from a rat lung scaffold to provide a molecular readout for tissue engineering. Mol Cell Proteomics. 2015 Apr;14(4):961-73. doi: 10.1074/mcp.M114.045260. Epub 2015 Feb 8. PubMed PMID: 25660013; PubMed Central PMCID: PMC4390273.

Personalized Medicine and Drug Development

• Liu C, D’Alessandro A, Xia Y. Metabolomics approach in probing drug candidates. Curr Topics Med Res 2016; invited review DOI : 10.2174/1568026617666161116144146

• D’Alessandro A, Zolla L. Proteomics, Metabolomics and Cancer Drug Development. Exp Rev Prot 2013; 10(5):473-88.

• D’Alessandro A, Zolla L. Metabolomics and cancer drug discovery: let the cells do the talking. Drug Discov Today 2012; 17(1-2):3-9.

• D’Alessandro A, Rinalducci S, Zolla L. Redox proteomics and drug development. J Proteomics. 2011; 74(12):2575-95

• D’Alessandro A, Zolla L. Pharmacoproteomics: a chess game on a protein field. Drug Discov Today. 2010;15(23-24):1015-23